what is another indicator found in dnase media

A hexahistidine tag is also attached onto the C-terminus of the composite to allow for easy purification of the expressed protein via metal-affinity column chromatography. We fused the DsbA 2-19 signal peptide sequence to the N-terminus of micrococcal DNase to with the aim of facilitating the fusion protein's export via the SRP pathway. It has recently been shown that the DsbA signal sequence is capable of mediating passenger protein secretion under a selection of different induction temperatures. The 2-19 peptide sequence of DsbA is a signal sequence that can direct passenger proteins for co-translational export via the signal recognition particle (SRP) pathway. ĭsbA is a thioredoxin fold-containing disulfide oxidoreductase protein found predominantly in Gram-negative bacteria, which functions as a protein-folding factor. Recently, micrococcal DNase has been shown to be able to inhibit the formation of bacterial biofilms. aeruginosa biofilms in cystic fibrosis for two decades now. It is a well-established fact that extracellular DNA is a vital structural component of bacterial biofilms, with aerosols of human recombinant DNase I having been employed as a remedy for P. Micrococcal DNase test agars are also a well-known indicator of S. As the enzyme's relatively simple structure proved to be very helpful towards the study of its biochemical and physical studies, researchers rapidly went on to identify the gene responsible for its synthesis and clone said gene in different expression hosts for heterologous characterization. The crystal structure of micrococcal DNase was resolved in 1971, long before the endogeneous function of said enzyme was discovered. Micrococcal DNase is an endo-exonuclease that non-specifically catalyzes the hydrolysis of single- and double-stranded DNA under basic conditions and in the presence of Ca 2+ ions, and is known to be able to speed up DNA hydrolysis by up to 10 16 times.

This part contains the sequence for the Staphylococcus aureus-derived enzyme, micrococcal DNase (also known as staphylococcal nuclease or thermonuclease) fused with an export signal at its N-terminus.īBa_K1659301 is a composite of Micrococcal DNase ( BBa_K1659300) with the 2-19 peptide segment of protein-folding factor DsbA: Micrococcal Nuclease (DNase) fused at N-terminal with DsbA signal sequence

What is another indicator found in dnase media